Abstract
Mahafacyclin B is a cyclic peptide isolated from the latex of Jatropha mahafalensis and is an antimalarial agent. However, the physiological effects of mahafacyclin B in mammalian cells are not known. Here, we assessed the growth, morphology, and alterations in the transcriptome of CHO-K1 cells exposed to mahafacyclin B (0–22 μM). Mahafacyclin B at 2.2 μM did not affect the proliferation or death of CHO-K1 cells. Mahafacyclin B was not toxic to mammalian cells at 2.2 μM, which represents a normal physiological concentration at which mahafacyclin B retains its antimalarial properties. Interestingly, mahafacyclin B altered the size and morphology of CHO-K1 cells. Comparative transcriptomics revealed that mahafacyclin B modulated the expression of a specific subset of genes.
Highlights
Cyclic peptides are polypeptide chains with a cyclic ring structure
We undertook gene-expression analyses of CHO-K1 cells exposed to mahafacyclin B
We estimated Shannon’s information entropy and the Kolmogorov complexity transcriptomic changes of CHO-K1 cells exposed to mahafacyclin B
Summary
Cyclic peptides are polypeptide chains with a cyclic ring structure. Cyclic peptides have antibacterial, immunosuppressive, and antitumor activities [1]. Cyclic peptides, owing to their increased stability, high resistance to exo-peptidases and endo-peptidases, enhanced binding affinity, and selectivity for target biomolecules, are commonly investigated as therapeutic agents [2]. Active cyclic peptides have been produced using biological and synthetic approaches, both of which have been adopted by pharmaceutical companies to develop novel medications. These novel medications represent a growing share of sales of all those produced worldwide [3]. Using a biological approach to develop medications includes a high level of risk (e.g. contamination with the porcine circovirus in human vaccines) [4], and sometimes the biological manufacturing process can affect public safety and health [5]. Several therapeutic cyclic peptides are derived from biological processing, but new methods used to synthesize cyclic peptides are under development [6]
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