Abstract

More and more attentions are paid to the potential effect of graphene oxide (GO) in environment and human beings. In order to evaluate the effect of GO on plant, tobacco BY-2 suspension cells were employed as material, and the physiological effect of GO on tobacco BY-2 suspension cells and its immigration were investigated. The results showed that low concentrations of GO (25 and 50 μg/mL) promoted cells growth (increased by 11.22 % in 50 μg/mL GO), while higher concentrations of GO (100 and 200 μg/mL) induced inhibition in cell growth (decreased by 9.68 % in 200 μg/mL GO). GO caused an increment in activity levels of SOD, POD and CAT, but the activity levels decreased with the extension of culture time in higher concentration. The results showed that GO could make cell nuclei fragment and loose in a higher concentration. These results imply that there is an adverse effect of GO on plant cells, and suggest that nano pollution should be paid attention to.

Highlights

  • Graphene oxide (GO) is one of the most important graphene derivatives and has been extensively studied in recent years [1]

  • The diffraction peak of exfoliated GO was found at 2 ~10.8° with crystalline orientation of (002) and interlay space (d-spacing) of ~0.82 nm

  • FTIR spectrum of GO showed that these distinctive peaks of O-H at 3401 cm-1, C=O at 1727 cm-1, C=C at 1604 cm-1, and C-O at 1045 cm-1 (Fig. 1(b))

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Summary

Introduction

Graphene oxide (GO) is one of the most important graphene derivatives and has been extensively studied in recent years [1]. While other studies on carbon nanomaterial-plants interactions showed that root growth of Brassica Juncea was enhanced up to 138 %, 202 % and 135 %, respectively, in the treatment of carbon nanotubes with concentrations of 10 μg/mL, 20 μg/mL and 40 μg/mL [7]. These reports about carbon nanomaterial-plant cell interaction can provide valuable reference to study the impact of GO on plant cells. PHYSIOLOGICAL EFFECT OF GRAPHENE OXIDE ON TOBACCO BY-2 SUSPENSION CELLS AND ITS IMMIGRATION.

Materials
Preparations and characterization of GO
Effect of GO on nucleus variation of tobacco BY-2 suspension cells
Characterization of GO
Effect of GO on cell growth and vitality of tobacco BY-2 cells
Effect of GO on nucleus variations of tobacco BY-2 suspension cells
Conclusions
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