Abstract

Summary The amount of repeated DNA nucleotide sequences present in the macronuclear genome of the ciliated protozoan, Tetrahymena pyriformis was determined for cells displaying several different cell generation times. Depending on the average cell generation time, the fraction of redundant DNA formed at a given Cot varies over a five-fold range (from 10 – 50%). Two separate Cot plots can be distinguished: one which yields a Cot½ of 400 for the less repeated fraction (SRF) and combines all the DNA preparations derived from cells whose average cell generation times are greater than 24 hours (infradian mode of growth), and one which has a Cot½ of 100 for the SRF fraction and which represents all the DNA preparations derived from cells dividing at a rate of more than once a day (ultradian mode of growth).

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