Abstract
Studies some of physiological properties on honey bee workers in both Carniolan and Italian pure strains in Order to reach a physiological method to differentiate between both strains and citrus honey reproduce from them with showed of DNA analysis of both strains by (PCR) polymerase chain reaction which indicated that: Deduce of Ca++ , Mg+++ , Na+ , K+ elements in citrus honey. Results were increase of Carniolan in the percentage of Ca++ and Mg +++, mean reached 23.44, 3.10 (mg%) and decreases in the Italian bee to reached 16.81, 2.01 (mg%), respectively. And were reached of Na+ and K+ 8.86 , 16.94 - 8,00 15.53 (m Eq%) of Italian and Carniolan bee, respectively. Increase of Carniolan bee of enzymes activities α and β esterase, Invertase, Trehalase and Amylase in reproduced citrus honey more than Italian bee. Increase of sugars (Glucose and Fructose) in field bee haemolymph in both strains bee. Increase of enzyme activities Invertase, Trehalase and Amylase in field bees haemolymph in both Carniolan and Italian strains. And not different between both strains in DNA analysis.
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