Abstract
The action of 7-bromo-5 (o-chlorophenyl) -3-propoxy-1,2-dihydro-3H-1,4-benzodiazepin-2-one (propoxazepam) with prolonged administration in various doses on behavioural reactions, anxiety and the memory of rats, as well as their muscle tone was estimated, which is important paying attention to its main (anticonvulsant and analgesic) pharmacological effects.The aim of the study was a comparative assessment of the severity and duration of propoxazepam effect after its administration to rats at doses of 2, 5 and 10 mg/kg (10 days) on higher functions of the central nervous system according to indicators such as motor and exploratory activity, as well as reference and working memory .Materials and methods. The study was conducted on 40 Wistar rats weighing 220-290 g. The psychophysiological state of the animals was evaluated using the open field test, the formation of spatial working and long-term memory in a eight-arm maze, muscle relaxation, imbalance and movements coordination - using the rotating rod method. Statistical processing was performed in Microsoft Excel 2016 with AtteStat 12.The results of the study. When comparing the test results of the open field, radial labyrinth and in groups of animals that were administered different doses of the compound, it was found that, in general, these parameters at doses of 2 mg/kg and 5 mg/kg statistically significantly different (increase) from control values, but are similar. At a dose of 10 mg/kg, most of the parameters (except for the rotarod test) for these animals were characterized by a downward trend.Conclusions. The administration of the drug in doses of 2, 5 and 10 mg/kg leads to a decrease in anxious behaviour of animals, which is also accompanied by a pronounced dose-dependent negative effect on the endurance, coordination and memory of animals. The administration of the drug at a dose of 10 mg/kg impaired the learning ability of animals and reduced memory function. This should be considered in clinical studies of the compound
Highlights
There are several approaches to studying animal behaviour
In previous studies [12], we proved that a single administration of propoxazepam to rats in moderate doses (0.15 and 1.5 mg / kg) did not cause significant changes in motor activity and tentative exploratory behaviour of animals, determined by the method of open field, in comparison with animals of the control group
Within the used doses (2-5–10 mg/kg), propoxazepam had a multidirectional effect on the behaviour of animals, with an increase in different types of activity along with a decrease in the number of fading, which is an indicator of reduced anxiety and the manifestation of a certain anxiolytic effect of the compound [14, 16]
Summary
There are several approaches to studying animal behaviour. Their use makes it possible to understand how behaviour helps an animal to adapt and survive in nature, or inherited an act of behaviour, or how the animal's nervous system works, or how the animal learns. It is believed that each type of activity of animals is carried out when reaching the optimal level of arousal. Both insufficient and excessive arousal reduce efficiency. It is hypothesized that the optimization of the level of excitation is a universal mechanism of animal behaviour [1]. This is quite successfully used in biomedical research, which makes it possible to find a way to treat a behavioural problem or, to find behaviour that could be judged on the pathology. In the biomedical sciences to clarify the indicators of such components of behaviour, there are appropriate terms: 1. Anxiolytic action – reducing feelings of emotional stress, depression, anxiety, fear, irritability and other manifestations of neurosis, psychopathic states and autonomic dysfunction
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