Physiological and Transcriptomic Analyses Revealed the Implications of Abscisic Acid in Mediating the Rate-Limiting Step for Photosynthetic Carbon Dioxide Utilisation in Response to Vapour Pressure Deficit in Solanum Lycopersicum (Tomato).

Dalong Zhang,Qingming Li,Po Sun,Min Wei,Jie Lou,Xiaotian Li,Qingjie Du

doi:10.3389/fpls.2021.745110

Abstract

The atmospheric vapour pressure deficit (VPD) has been demonstrated to be a significant environmental factor inducing plant water stress and affecting plant photosynthetic productivity. Despite this, the rate-limiting step for photosynthesis under varying VPD is still unclear. In the present study, tomato plants were cultivated under two contrasting VPD levels: high VPD (3–5 kPa) and low VPD (0.5–1.5 kPa). The effect of long-term acclimation on the short-term rapid VPD response was examined across VPD ranging from 0.5 to 4.5 kPa. Quantitative photosynthetic limitation analysis across the VPD range was performed by combining gas exchange and chlorophyll fluorescence. The potential role of abscisic acid (ABA) in mediating photosynthetic carbon dioxide (CO2) uptake across a series of VPD was evaluated by physiological and transcriptomic analyses. The rate-limiting step for photosynthetic CO2 utilisation varied with VPD elevation in tomato plants. Under low VPD conditions, stomatal and mesophyll conductance was sufficiently high for CO2 transport. With VPD elevation, plant water stress was gradually pronounced and triggered rapid ABA biosynthesis. The contribution of stomatal and mesophyll limitation to photosynthesis gradually increased with an increase in the VPD. Consequently, the low CO2 availability inside chloroplasts substantially constrained photosynthesis under high VPD conditions. The foliar ABA content was negatively correlated with stomatal and mesophyll conductance for CO2 diffusion. Transcriptomic and physiological analyses revealed that ABA was potentially involved in mediating water transport and photosynthetic CO2 uptake in response to VPD variation. The present study provided new insights into the underlying mechanism of photosynthetic depression under high VPD stress.

Highlights

Carbon dioxide (CO2) is significant for plant photosynthesis, growth, and yield production
Vapour pressure deficit significantly affected the distribution of water potential along the soil-plant-atmospheric pathway (Figure 1)
Atmospheric evaporative demand increased with vapour pressure deficit (VPD) elevation, which triggered plant water stress and a linear decline in the leaf water potential (Figure 1A)

Summary

Introduction

Carbon dioxide (CO2) is significant for plant photosynthesis, growth, and yield production. CO2 fertilisation and globally elevated trends are expected to improve crop photosynthesis and yield, large evidence has shown that the magnitude of such enhancement is constrained by other climate change-derived phenomena, such as more extreme and frequent environmental stress (Norby, 2002). The bottlenecks constraining the CO2 utilisation efficiency are limited CO2 acquisition and assimilation. There is increasing evidence from physiology and crop production that high vapour pressure deficit (VPD) induces plant water stress and inhibits photosynthetic productivity (Lu et al, 2015; Zhang et al, 2015). Few previous studies have quantitatively addressed the components of photosynthetic limitation across a series of VPD. The rate-limiting step for photosynthetic CO2 transport and utilisation under different VPD conditions was highly uncertain. A quantitative limitation analysis consisting of stomatal, mesophyll, and biochemical limitations is essential to reveal the underlying mechanism by which the VPD affects the photosynthetic process

Methods

Results

Conclusion