Abstract
Sorbus pohuashanensis (Hance) Hedl. is a Chinese native alpine tree species, but the problem of introducing S. pohuashanensis to low altitude areas has not been solved. In this study, we aimed to explore the molecular regulatory network of S. pohuashanensis in response to high-temperature stress using RNA-Sequencing technology and physiological and biochemical determination. Based on transcriptomic data, we obtained 1221 genes (752 up-regulated and 469 down-regulated) that were differentially expressed during 8 h 43℃ treatment and candidate genes were related to calcium signaling pathway, plant hormone signal transduction, heat shock factors, chaperones, ubiquitin mediated proteolysis, cell wall modification, ROS scavenging enzymes, detoxification and energy metabolism. The analysis of high temperature response at the physiological level and biochemical level were performed. The chlorophyll fluorescence parameters of leaf cells decreased, the content of osmotic regulators increased, and the activity of ROS scavenging enzymes decreased. The molecular regulatory network of S. pohuashanensis in response to high-temperature stress was preliminarily revealed in this study, which provides fundamental information improving introducing methods and discovering heat-tolerant genes involved in high-temperature stress in this species and provides a reference for other plants of the genus Sorbus.
Highlights
Sorbus pohuashanensis (Hance) Hedl. is a Chinese native alpine tree species, but the problem of introducing S. pohuashanensis to low altitude areas has not been solved
PSII of S. pohuashanensis leaves in high-temperature treatment group (HT) showed a downward trend with the extension of time under high temperature stress, which was significantly lower than CK after 4 h of high temperature stress (Fig. 1c)
There was no difference in qL between HT and CK in the first 6 h of high temperature stress, and HT was significantly lower than CK at 6–8 h of high temperature stress (Fig. 1d)
Summary
Sorbus pohuashanensis (Hance) Hedl. is a Chinese native alpine tree species, but the problem of introducing S. pohuashanensis to low altitude areas has not been solved. The molecular regulatory network of S. pohuashanensis in response to high-temperature stress was preliminarily revealed in this study, which provides fundamental information improving introducing methods and discovering heat-tolerant genes involved in high-temperature stress in this species and provides a reference for other plants of the genus Sorbus. Plant response to high-temperature stress regulation network including small G protein/G protein mediated calcium signaling pathways, hormone regulation networks, heat shock transcription factors (Hsfs) and heat shock proteins (Hsps) reaction and reactive oxygen species (ROS) reaction, and these factors interact together to complete the response of high-temperature stress[15]. The differentially expressed genes (DEGs) of S. pohuashanensis were analyzed and the molecular regulatory networks were constructed during high-temperature treatment using RNA-seq technology to reveal the molecular response mechanisms to high temperature with determination of chloroplast fluorescence parameters, osmotic regulation substance content, and ROS scavenging enzyme activity. The results will contribute to the development and utilization of germplasm resources for S. pohuashanensis and provide an informatics basis for the genetic improvement and breeding of varieties of this species in the future
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