Physiological and Photosynthetic Characteristics of Pumpkin Seedlings Cultivated Under Low-Light Stress

Abstract

Low-light stress is known to have a significant impact on pumpkin yield in greenhouse conditions, it is crucial to understand the characteristics of low-light tolerance and identify resistant germplasm resources. In this study, four pumpkins (Cucurbita moschata) varieties were used, including three inbred lines (10–32, NY-1, and JB-1) and one hybrid line (NY-1×JB-1). Various low-light treatments (T0, no shade; T40, 40% shading; T80, 80% shading) were set, to explore their effects on morphology, photosynthetic characteristics, osmotic regulation substance content, and antioxidant enzyme activity in pumpkin seedling leaves. In the results, seedlings of inbred lines 10–32 and JB-1, as well as hybrid NY-1×JB-1, exhibited low-light resistance with shading rate increases. The maximum photochemical quantum yield of photosystem II (Fv/Fm) of all varieties decreased under low-light stress. Catalase (CAT) activity initially increased and then decreased, while superoxide dismutase (SOD) activity showed the opposite trend. Peroxidase (POD) activity gradually increased in 10–32, JB-1, and NY-1×JB-1, with JB-1 showing a significant increase at T80, and NY-1 displaying an initial decrease followed by an increase. Noteablely, the soluble proline (Pro) content decreased in 10–32, NY-1, and NY-1×JB-1. Significant differences in malondialdehyde (MDA) content were observed among the seedlings of four varieties. The MDA contents increased in 10–32 and JB-1 under T0 and T40, while showed no significant change in NY-1. The MDA content at T80 significantly decreased in NY-1, and first decreased and then significantly increased in NY-1×B-1. The soluble sugar content in 10–32 remained unchanged between T0 and T40, but significantly increased at T80. While in JB-1 and NY-1, it initially increased then decreased with increasing shading rate. The trend in NY-1×JB-1 was opposite to that of JB-1 and NY-1, with initially decreased followed by a significant increase. In conclusion, morphological characteristics associated with low-light tolerance can serve as reference indices for breeding low-light tolerance pumpkins. These parameters can be used as reference indices to assess pumpkin resistance to low-light stress.