Physiological and Molecular Responses of Camellia oleifera Seedlings to Varied Nitrogen Sources

Abstract

Camellia oleifera Abel. is a unique woody edible oil tree species in China mainly distributed in red soil areas. Nitrogen is one of the important growth-limiting factors for C. oleifera, and the nitrogen form has a vital impact on the growth and development of the plants. Ammonium and nitrate are the two nitrogen forms that are maximally absorbed and utilized by the plants. Here, we used one-year-old seedlings of the main varieties of C. oleifera (‘Xianglin No. 1’, ‘Xianglin No. 27’, and ‘Xianglin No. 210’) to set up six experimental groups treated with different nitrogen forms ([NO3−:NH4+] 0:0, 10:0, 7:3, 5:5, 3:7, and 0:10) and investigated the effects of nitrogen on the vegetative growth and photosynthetic characteristics of the seedlings. This study showed that mixed nitrogen sources could significantly increase the seedling height, ground diameter, biomass accumulation, and photosynthesis efficiency. Transcriptome sequencing analysis led to the identification of 3561 differentially expressed genes in the leaves. Preliminary screening identified several key enzyme genes from nitrogen nutrient metabolic pathways that were differentially expressed among seedlings grown with different nitrogen forms, and their expression pattern changes were further characterized. All the results demonstrate that the same proportion of ammonium nitrate ratio promoted the expression of genes encoding glutamine synthetase and glutamate synthase, thereby improving nitrogen assimilation and utilization efficiency. This study could provide a theoretical basis for scientific and rational fertilization and the improvement of nitrogen utilization efficiency in C. oleifera seedlings.