Physiological and Histopathological Effects of Nettle Seed (Urtica Pilulifera), Grape Seed (Vitis Vinifera), Flax Seed (Linum Usitatissimum) in Broiler.

Abstract

Objective: In this study, it was aimed to investigate the effects of nettle seed, grape seed and flax seed added to the broiler diet on body weight, electrocardiogram, hematological and histopathological parameters. 
 Materials and Methods: In this study 40 daily Ross 308 female broilers were used. Chicks were divided into 4 groups of 10 chicks each. During 42 days, in addition to standard broiler feed, nettle seed, grape seed and flax seed with 30 gr/kg/day were added to group II, III, IV, respectively. The values of erythrocyte (RBC), leukocyte (WBC), platelet (PLT), hematocrit (HCT), hemoglobin (Hb) and percentages of white blood cell were determined by conventional methods. The liver, kidney, spleen and lung tissues held in formaldehyde (10%), were passed through the alcohol and xylose serial in routine tissue tracking and were buried in paraffin blocks. They were prepeared on the lam and stained with Hematoxylin-eosin (HE) and examined by light microscopy. 
 Results: It was determined that body weight was higher in control group than those in the other groups in the second weighing and there was a decrease in the number of heart breath in the group IV (p≤0.05). There was no significant difference with regard to hematological and histopathological findings.
 Conclusion: Even if the additionally nettle seed, grape seed and flax seed (30 mg/kg) to broiler rations did not have any positive effect on body weight gain, it is also important that it has no negative effect on blood, heart, liver, kidney, lung and spleen.