Physiological and growth responses of Cedrus libani seedlings to cold storage

Abstract

The effects of cold storage duration on the physiological characteristics and growth of two-year-old Taurus cedar (Cedrus libani A. Rich) seedlings were studied. Taurus cedar seedlings were lifted in December, January and February and stored at +4 °C (cold storage) for 0, 2, 3 and 4 months. Xylem water potential (Ψ), shoot (SMC) and root moisture (RMC) contents, root growth potential (RGP), root electrolyte leakage (REL) and total carbohydrate contents were determined before and after the cold storage. The survival and growth were also evaluated at the end of the first growing season. Ψ, SMC and RMC, RGP and total carbohydrate contents were dramatically affected by the storage duration. The decrease in total carbohydrate contents during the storage showed a parallelism with RGP and survival. It was also found that storage duration had important effects on survival and growth. While survival was above 85 % even after storage of 4 months in seedlings that were lifted in December and January, this rate was reduced to 30 % after storage of 4 months in seedlings that were lifted in February. Total carbohydrate content and RGP can be used as an indicator of survival after cold storage.