Physiological and genetic diversity analysis of diploid and autotetraploid Platycodon grandiflorm A. De Candolle

Abstract

This study was carried out to investigate the DNA markers between diploid and colchicine-induced autotetraploid Platycodon grandiflorum A. De Candolle (P. grandiflorum A. DC) plants using sequence-related amplified polymorphism (SRAP) analysis, amplified fragment length polymorphism analysis (AFLP). Autotetraploid P. grandiflorum A. DC plants were induced using colchicine. In comparison with diploid plants, autotetraploid P. grandiflorum A. DC plants showed lower plant height, stomata density, and MDA content (p < 0.05); higher leaf length and width, stomata width and length, SOD and POD activity, soluble sugar and protein content, and total chlorophyll content (p < 0.05). Using SRAP and AFLP analysis, we identified 23 (11.16%) and 13 (2.73%) polymorphic bands in autotetraploid P. grandiflorum A. DC plants in comparison with diploid plants, respectively. SRAP and AFLP markers are related to diversities in morphology, biochemistry, and oxidative activity between diploid and autopolyploid P. grandiflorum plants.