Abstract
Fructose is toxic for Synechocystis PCC 6714 and 6803, strains which grow chemoheterotrophically on glucose. This toxicity, as well as fructose uptake, were inhibited by glucose or by its non-metabolized analogue 3-O-methyl-glucose. The results suggested that both sugars were transported by the same permeation system, the affinity for fructose, estimated from the corresponding Km and Ki, being very low. The unicity of the permeation system was further established by the isolation of spontaneous mutants showing the expected pleiotropic phenotype, Glu−, Frur, transport−, and by the simultaneous re-acquisition of the relevant wild type characteristics in mutant cells transformed by wild type DNA. The genetic nature of this mutation is discussed in view of the impossibility to isolate spontaneously reversed wild type clones from the transport deficient mutants.
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