Physiological and biochemical response of P. fortunei to Mn exposure.

Abstract

Fast-growing woody plants with metal tolerance are considered as potential candidates for phytoremediation. P. fortunei is widely distributed in China. Herein, the Mn tolerance ability and physiological and biochemical response of P. fortunei to Mn were explored in this study. Results showed that a low concentration of Mn exposure was favorable for the growth of P. fortunei, while it was inhibited in high Mn exposure. P. fortunei showed high tolerance to Mn (10mmol/L). The microstructure of P. fortunei organs revealed that the Mn tolerance of P. fortunei was related to the compartmentalization of the cell wall. The subcellular distribution of Mn in P. fortunei showed that Mn was mainly stored in the cell wall fraction (39%-90%). Under Mn exposure, the proportion of pectate and protein-integrated Mn increased by 5%-29% in P. fortunei. The changes of function groups (-CH3 and -COOH) in P. fortunei might be related to the reduction of Mn toxicity in plant cells in the way of chelation. Additionally, P. fortunei leaves resisted Mn toxicity by increasing the activities of CAT and SOD under low Mn concentration exposure, but it might be destroyed under excessive Mn concentration exposure. P. fortunei might be used as a candidate plant for low concentration Mn tailing restoration.