Physiological and biochemical markers of gamma irradiated white radish (Raphanus sativus)

Abstract

Purpose A field experiment was performed to investigate the impact of low-dose gamma rays on growth parameters and bioactive compounds of white radish. Materials and methods White radish seeds were irradiated by gamma rays dose levels (10, 20, 40 and 80 Gy) beside control. Physiological and biochemical markers were done to follow the effect of gamma rays on white radish. Results The results revealed that gamma rays increased growth parameters with increasing irradiation to a dose of 40 Gy. The maximum increments were found at 14.64 (cm), 48.30 (cm), 20.84 (cm) and 5.51 (cm) for leaves number, leaves length, roots length and roots diameter, respectively, with a dose of 40 Gy. By increasing the irradiation dose to 80 Gy, the results showed reduction in all parameters studied. Ascorbic acid gave the maximum increase with the dose of 40 Gy, while phenols, flavonoids, antioxidant activity, peroxidase, and polyphenol oxidase showed the highest increase with the dose 80 of Gy in radish leaves. Similar trend was observed for the radish roots. Furthermore, the protein and isoenzyme profiles of peroxidase and polyphenol oxidase changed and induced alteration by different irradiation dose levels. Conclusion Gamma rays can be a useful tool for increasing the growth and biochemical content of white radish plants and perhaps other food crops.