Abstract
Management of solid wastes with high nicotine content, such as those accumulated during tobacco manufacturing, poses a major challenge, which can be addressed by using bacteria such as Pseudomonas and Arthrobacter. In this study, a new species of Pseudomonas geniculata, namely strain N1, which is capable of efficiently degrading nicotine, was isolated and identified. The optimal growth conditions for strain N1 are a temperature of 30°C, and a pH 6.5, at a rotation rate of 120 rpm min−1 with 1 g l−1 nicotine as the sole source of carbon and nitrogen. Myosmine, cotinine, 6-hydroxynicotine, 6-hydroxy-N-methylmyosmine, and 6-hydroxy-pseudooxynicotine were detected as the five intermediates through gas chromatography-mass and liquid chromatography-mass analyses. The identified metabolites were different from those generated by Pseudomonas putida strains. The analysis also highlighted the bacterial metabolic diversity in relation to nicotine degradation by different Pseudomonas strains.
Highlights
Nicotine, a principal pyridine alkaline in tobacco plants, is notorious for its significant contribution to tobacco addiction
This study suggests that the nicotine-degrading bacterium has future potential application on the treatment of the waste generated during tobacco manufacturing
Strain N1 could utilize a narrow range of carbon sources such as polychrom, and it grew weakly in glucose, amygdalin, arbutin, and saligenin (Table 1)
Summary
A principal pyridine alkaline in tobacco plants, is notorious for its significant contribution to tobacco addiction. Nicotine is very toxic to humans because it is absorbed in the body; its hydrophilic nature contributes to the environmental contamination [1]. Large quantities of tobacco wastes containing high concentration of nicotine are produced during tobacco manufacturing process. These wastes have been classified as ‘‘toxic and hazardous wastes’’ by European Union Regulations [2]. The American Medical Association has issued a public strategy strengthening the forcible reduction of nicotine level in tobacco [3]. Microbial degradation of nicotine has been considered as a promising method due to its low cost and high efficiency
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