Abstract

1. 1. An analysis of the physiological (free) amino acids in the central nervous system of the tarantula spiders, Aphonopelma chalcodes and Dugesiella echina (Theraphosidae) was conducted. 2. 2. Nitrogenous waste products (ammonia and urea) together comprised 56% of the total ninhydrin-positive compounds in A. chalcodes and 55% in D. echina. 3. 3. GABA accounted for 6% of the total ninhydrin-positive compounds in the CNS of A. chalcodes, and 7% in D. echina. 4. 4. GABA, aspartate, taurine, glycine and glutamate (which have been shown to function as neurotransmitters and/or comodulators of NT activity) together comprised 25% of the total ninhydrin-positive compounds. 5. 5. The most abundant amino acids were proline, alanine, glutamate and aspartate. Glutamate and aspartate concentrations in the CNS of the more primitive theraphosids are lower than those found in web-building spiders.

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