Abstract
In order to develop the new physiologically active materials from the leaves of Panax ginseng C.A. Meyer, the ginsenoside-rich fraction (GL-1) was isolated from ginseng leaves and physiological activities were examined. GL-1 contained crude saponin (ginsenosides, 58.00%) and component ginsenoside analysis using high performance liquid chromatography (HPLC) showed that the major ginsenosides of GL-1 were Re, Rg1, Rc, and F2 and their contents were 34.42, 20.73, 18.42, and 8.60%, respectively. ABTS and DPPH radical scavenging activities of GL-1 were 75.14 and 39.28% at 10,000 μg/mL, respectively. Superoxide anion radical scavenging activity was 70.94% at 1,000 μg/mL. GL-1 also expressed the considerable anti-complementary activity in dose-dependent manner. Results obtained by crossed immunoelectrophoresis using anti-human C3 and anti-complementary activity in the absence of Ca++ ion suggested that complement activation by GL-1 from ginseng leaves occur via both alternative and classical pathways.
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