Abstract
The effect of microbial transglutaminase (MTGase, EC 2.3.2.13) on type I collagen self-assembly and the physicochemical property of MTGase-crosslinked pig collagen gel was investigated. The rate of collagen self-assembly, especially the formation of nucleus for collagen fibril was accelerated with the addition of MTGase. MTGase-crosslinked collagen gel was characterized by non-destruction test of dynamic light scattering. Since diffusion coefficient was found to decrease, it was suggested that the spacing of MTGase crosslinked collagen gel became wide. MTGase-crosslinked collagen gel showed lower mechanical strength and about 2°C higher denaturation temperature than that of control. And MTGase-crosslinked collagen gei was not melt at 52°C instantly. The introduction of crosslinkage into collagen with MTGase resulted in the acceleration of collagen self-assembly and the improvement of thermostability of collagen gel.
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