Abstract
The physicochemical properties of renin substrate in plasma from normal estrogen-treated and nephrectomized male rats were determined. The molecular radii molecular weights net charge and isoelectric points of substrates from 5 normal rats 5 rats 24 hours postnephrectomy and 5 rats 24 hours after intravenous injection of 100 mcg ethinyl estradiol were determined on polyacrylamide gels by the Rodbard and Chrambach technique. Plasma angiotensin 1 liberated by an excess of renin and measured by radioimmunoassay was 1200 ng/ml in normal rat 2300 ng/ml in estrogen-treated rat and 8000 ng/ml in binephrectomized rat. The 3 substrates revealed the same relative mobility. Their molecular radii were identical (r=2.23 mmc) and sizes (molecular weight=37200-40800) and isoelectric points (pI=4.79-4.83) were similar. It was concluded the estrogen treatment and binephrectomy increase the amount of available plasma renin substrate but do not modify its physicochemical properties.
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