Abstract
Bovine high density lipoprotein, floating between densities of 1.063 g per ml and 1.125 g per ml, constitutes about 80% of all bovine serum lipoproteins. Preparations of this lipoprotein appear homogeneous by several criteria: electrophoresis, sedimentation velocity, sedimentation equilibrium, and NH2-terminal amino acid analysis. Bovine high density lipoprotein is a spherical protein-lipid aggregate, containing 32% protein and 68% lipid by weight; its molecular weight is 376,000. The protein component appears to consist of 4 subunits of molecular weight 28,100. Tryptophan fluorescence spectra of the native lipoprotein and of its delipidated form have maximum fluorescence wave lengths at 328 and 338 nm, respectively, an indication that on delipidation the environment of tryptophan residues becomes more polar.
Highlights
This paper describes the isolation and characterization of a dominant high density lipoprotein from bovine serum
Bovine Lipoprotein Classes-Ultracentrifugal flotation patterns were obtained for lipoproteins from three animals, each sample was run at three densities: 1.063, 1.125, and 1.200 g per ml
Lipoproteins which floated between densities 1.063 and 1.125 g per ml and 1.063 and 1.200 g per ml were identical; there appeared to be no lipoproteins of densities higher than 1.125 g per ml in significant amounts
Summary
Preparation of Bovine Lipoproteins-Blood for the preparation of bovine lipoproteins was obtained from jugular puncture of three healthy heifers. The blood, which was collected directly into centrifuge bottles was allowed to stand at room temperature for 3 to 4 hours to insure complete clotting. Serum was decanted after centrifugation at 1500 rpm for 15 min. All lipoprotein preparations were performed within 3 weeks from the drawing of blood. Preparations of lipoprotein fractions for ultracentrifugal Aotation analyses were carried out essentially by the procedure of de Lalla and Gofman [15]. Stock solutions of NaCl and NaBr of high densities were used to dilute bovine serum to final background densities of 1.063, 1.125, and 1.200 g per ml. Analysis (GLICK, D., ed) Interscience, New York.
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