Abstract
ABSTRACTPepsin-soluble collagen was successfully prepared from the body wall of starfish (Asterina pectinifera) (PSCBWS). Amino acid composition suggested that the collagen might be classified as type I collagen. Ultraviolet-visible and Fourier transform infrared spectroscopic analyses showed that the PSCBWS was a high-purity collagen that maintained the intact triple-stranded helices. Physical and chemical characterizations of the PSCBWS showed an isoelectric point (pI) of 5.0 ± 0.2, superior moisture-absorption and retention capacities compared with glycerol, a minimum solubility at pH 5.0 in 0.5 M acetic acid, and a sharp decrease in solubility in the presence of low concentration of NaCl. The PSCBWS was evaluated for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and hydroxyl radicals. Results indicated that PSCBWS possessed DPPH and hydroxyl radicals scavenging capacity in a dose-dependent manner. These results indicated that PSCBWS might be used as a new collagen resource in the food and cosmetic industries.
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