Abstract
Glass surfaces, although the first to be used for culturing ex vivo adherent cells, are not the perfect substrates for this purpose. Today, plastics dominate these applications, but in light of the global trend to reduce the use of synthetic polymers, it is reasonable to consider a return to glass vessels with coatings for these purposes. The ideal surface for cell growth is one that simulates the composition and structure of the mainly protein-based intercellular matrix. The work presented here shows a new idea of preparing porous protein coatings on glass using biosynthesis. The process utilizes the colonization of the gold nanoparticle-coated glass surface with Lactobacillus rhamnosus GG bacteria, followed by permeabilization (using ethanol) of their membrane and partial thermal degradation (at 160 °C in vacuum) of the surface-bound protein components of these microorganisms. It results in a development of coating on the glass that promotes mammalian cell growth, which has been preliminary confirmed using Vero cells. Subsequent steps in the formation of coating components were documented by reflectance ultraviolet and visible spectra and infrared spectroscopy. The presence of microorganisms and mammalian cells was confirmed using scanning electron and optical microscopy and crystalline violet staining.
Highlights
Cell line cultures are one of the most important tools of modern biological and medical sciences [1,2]
Central to the proposed new strategy is our observation described in earlier publications regarding the phenomenon of Lactobacillus rhamnosus GG bacterial growth on gold surfaces [11,12]
We show in this work that even a small modification, in terms of the amount of material, caused by adsorption of gold nanoparticles via –SH groups [20,21] to the glass surface, is enough to make this substrate susceptible to colonization by Lactobacillus rhamnosus GG bacteria
Summary
Cell line cultures are one of the most important tools of modern biological and medical sciences [1,2]. For more demanding cell lines, modified polystyrene is not sufficient and additional coatings of collagen, gelatin or polylysine are used [7] These solutions are effective, but usually labor intensive and use perishable protein materials. As in the case of plastic, or more precisely, polystyrene (oxidized surface [5]), where the surface coating improved the properties of vessels, a similar effect can be expected for glass. Such coatings would have to be relatively easy and “green” to produce and, at the same time, stable and facile to sterilize. The obtained results, they should be considered as preliminary because the study was performed on only one line, are very promising and show that the new coating favors the growth of cells on the surface compared to glass and does not affect their morphology
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