Abstract
The purpose of this work was to evaluate the physicochemical properties, the cytotoxicity and in vivo biocompatibility of MTA Repair HP (MTA HP) and White MTA (WMTA). The setting time, flow, radiopacity and water solubility were assessed. To the cytotoxicity assay, primary human osteoblast cells were exposed to several dilutions of both materials eluates. MTT assay, apoptosis assay and cell adhesion assay were performed. The in vivo biocompatibility was evaluated through histological analysis using different staining techniques. No differences were observed between MTA HP and WMTA for setting time, radiopacity, solubility and water absorption (P > 0.05). However, MTA HP showed a significantly higher flow when compared to WMTA (P < 0.05). Cell viability results revealed that the extracts of WMTA and MTA HP promoted the viability of osteoblasts. After incubation of cells with the endodontic cement extracts, the percentage of apoptotic or necrotic cells was very low (<3%). Furthermore, SEM results showed a high degree of cell proliferation and adhesion on both groups. MTA HP showed similar in vivo biocompatibility to the WMTA and the control group in all time-points. The MTA HP presented adequate physicochemical and biological properties with improved flow ability when compared to WMTA. Such improved flow ability may be a result of the addition of a plasticizing agent and should be related to an improvement in the handling of MTA HP.
Highlights
The purpose of this work was to evaluate the physicochemical properties, the cytotoxicity and in vivo biocompatibility of Mineral trioxide aggregate (MTA) Repair HP (MTA HP) and White MTA (WMTA)
No differences were observed between MTA Repair HP and White MTA for setting time (p = 0.0921), radiopacity (p = 0.1198), solubility (p = 0.8538) and water absorption (p = 0.2856)
MTA Repair HP showed a significantly higher flow when compared to White MTA (p = 0.0001)
Summary
The purpose of this work was to evaluate the physicochemical properties, the cytotoxicity and in vivo biocompatibility of MTA Repair HP (MTA HP) and White MTA (WMTA). The MTA HP presented adequate physicochemical and biological properties with improved flow ability when compared to WMTA. Such improved flow ability may be a result of the addition of a plasticizing agent and should be related to an improvement in the handling of MTA HP. The use of bismuth oxide as a radiopacifying agent may promote discoloration of the tooth and marginal gingiva[5,6] and affect the physicochemical properties of this material[5,7]. The main differences to its predecessor are the addition of an organic plasticizer to the distilled water (promoting greater plasticity to the material) and the exchange of bismuth oxide by calcium tungstate as a radiopacifying agent. Calcium tungstate has been shown to be more biocompatible than bismuth oxide[11]
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