Abstract
Phage N5 is one of the phages of Vibrio cholerae serovar O1 biotype El Tor (Ghosh, A. N., Ansari, M. Q., and Dutta, G. C. Isolation and morphological characterization of El Tor cholera phages. J. Gen. Virol. 70: 2241–2243, 1989). In the present communication the growth curve, molecular weight and confirmation of the genome, partial denaturation map and restriction endonuclease digestion pattern have been determined. Partial denaturation map indicates that the genome has non-permuted / invariant sequence. Presence of cohesive ends has also been documented.
Highlights
The N5 phage was isolated from the sewage water of Calcutta [3] and was propagated on MAK 757, a Vibrio cholerae O1 ElTor strain
In the present work we report physicochemical characterization of an ElTor vibriophage N5 that was isolated from the sewage water samples of Calcutta, India [3]
The phage pellet was resuspended in 1 ml of 50 mM – Tris-HCl pH 7.5, 20 mM – MgCl2 (TM buffer) to concentrate and the phage was stored at 4°C
Summary
Principles and practice of typing Vibrio cholerae. 2. Guidolin A, Manning PA: Genetics of Vibrio cholerae and its bacteriophages. 3. Ghosh AN, Ansari MQ, Dutta GC: Isolation and morphological characterization of El Tor cholera phages.
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