Abstract
To investigate the structure and immunomodulatory activity of polysaccharide from Solanum muricatum, a novel acid polysaccharide named SMP-3a was purified from Solanum muricatum pulp through DEAE-52 cellulose column and Sephadex G-200 chromatography. Monosaccharide composition analysis showed that SMP-3a was mainly composed of rhamnose, arabinose, galactose, and galacturonic acid with the molar ratio of 1.09:2.64:1.54:1. The average molecular weight was found to be 227 kDa by high performance gel permeation chromatography (HPGPC). Thermal studies revealed the SMP-3a was a thermally stable polymer. Based on the results of methylation and NMR analysis, the backbone chain of SMP-3a was composed of →2)-α-l-Rhap-(1→, →4)-α-d-GalpA-(1→ and →4)-α-d-Galp-(1→. The side chain was consisted of α-l-Araf-(1→ and →5)-α-l-Araf-(1→. Immunomodulatory assay indicated that SMP-3a could significantly promote the proliferation of macrophages and stimulate the secretion of cytokines, including TNF-α, IL-1β, and IL-6. Our results suggested that SMP-3a could be used as a novel potential immunomodulatory agent in functional food.
Highlights
Solanum muricatum, which belongs to the Solanaceae family, is an herbaceous plant originally cultivated in the Andean region of South America and has been disseminated throughout the world.It is commonly called pepino or melon pear and can be grown under diverse soil and climatic conditions [1,2]
SMP was purified by DEAE-52 anion-exchange chromatography with various concentrations of NaCl solutions
Structural analysis revealed that SMP-3a was mainly consisted of rhamnose, arabinose, galactose, and galacturonic acid with the molar ratio of 1.09:2.64:1.54:1
Summary
Solanum muricatum, which belongs to the Solanaceae family, is an herbaceous plant originally cultivated in the Andean region of South America and has been disseminated throughout the world.It is commonly called pepino or melon pear and can be grown under diverse soil and climatic conditions [1,2]. Solanum muricatum, which belongs to the Solanaceae family, is an herbaceous plant originally cultivated in the Andean region of South America and has been disseminated throughout the world. The color of Solanum muricatum begins green and changes to golden yellow covered with purple stripes during the ripening period [3] This fruit is rich in minerals such as calcium, potassium, and phosphorous. The analysis of volatile aromatic constituents of Solanum muricatum have shown that Solanum muricatum contains terpenes and β-damascenone, which contributes to the exotic aromas of the food [5] This plant food is rich in phenolic acids and flavonoids [6]
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