Abstract
Waste of parang parang fish (Chirocentrus dorab) skin can be used as a source of collagen. Collagen isolation can be done chemically by the Acid Soluble Collagen (ASC) method. The objective of this research was to isolate collagen with ASC method and characterize their physicochemical. Collagen isolation consisted of pretreatment and hydrolysis with acids. The pretreatment used NaOH 0.1 M for 12 hours, while hydrolysis used acetic acid 0.5 M. Pretreatment results indicated that the concentration of non-collagen protein was 0.1243 mg/mL, while the yield collagen was 2.61%. The collagen had the viscosity of 6.50 cP, the denaturation temperature of 4°C, the transition temperature of 77.30°C, and the melting temperature of 153.90°C. The obtained collagen also had pH of 6.25. The fourier transform infrared (FTIR) spectra analysis showed the collagen contained amide A (3425.58), B (2924.09), I (1647.21), II (1543.05), and III (1246.02) (cm-1). The collagen also contained glycine (26.69%), proline (12.24%) and alanine (9.51%).
Highlights
Waste of parang parang fish (Chirocentrus dorab) skin can be used as a source of collagen
The collagen had the viscosity of 6.50 cP, the denaturation temperature of 4°C, the transition temperature of 77.30°C, and the melting temperature of 153.90°C
Hasil isolasi kolagen kulit ikan parang parang metode kolagen larut asam terdapat amida A pada puncak serapan 3425,58 cm-1 dengan wilayah serapan (33503550 cm-1) yang memiliki karakteristik adanya renggangan NH (Erizal et al 2012)
Summary
Bahan yang digunakan dalam penelitian ini adalah kulit ikan parang-parang yang didapat dari Pasar Anyar Bogor. Kulit ikan parang parang dibersihkan kemudian dimasukkan coolbox (stereofoam) dan diberi es. Sampel diterima di laboratorium dalam keadaan dingin (10°C). Bahan kimia yang digunakan adalah akuades, NaOH 0,1 M (sigma aldrich), asam asetat (CH3COOH 80%) (Sigma Aldrich), Bovine Serum Albumin (BSA) (sigma Aldrich), dan reagen Bradford (Sigma Aldrich). Alat-alat yang digunakan adalah Fourier Transform Infrared (FTIR) Spectroscopy (Bruker Tensor 37 German), spektrofotometer (Genesys 10 UV Thermo Scientific), viskometer (Brookfield), kromamater (Lutron RGB 1002), dan termal differential scanning calorimatry (DSC-60 Shimadzu), neraca analitik (FS-Ar 210), pH meter (Hanna Instruments), freeze dry (Christ Alpha 2-4 LD UK), UPLC (ELSD Waters Acquity H-Class)
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