Abstract
Mycoremediation is the application of fungi isolate to contaminated sites. The mycological content of Ejamah- Ebubu oil polluted site was carried out. Using composite sampling technique, five sets of samples were collected; At15, Bt15, Ct15, Dt15, Et15 at depth (0 - 15cm) and Ab30, Bb30, Cb30, Db30, Eb30 at depth (15 - 30cm). The parameters analyzed include; pH, conductivity, nitrate, phosphate, sulphate, total heterotrophic fungal count (THF) and total hydrocarbon utilizing fungal count (HUF). The total heterotrophic fungi and hydrocarbon utilizing fungal count for A15 ranges between 5.0 x 10 3- 1.5x104cfu/g and 1.1 x103 - 2.3x103 cfu/g while A30 ranges between 4.0 x 103- 1.3x104cfu/g and 3.0 x 103 - 1.3x103cfu/g. A total of nine fungal isolate were obtained and identified to belong to the genera: Aspergillus (44.44%), Microsporum (11.11%), Fusarium (11.11%), Penicillium (22, 22%) Acremonium (11.11%). The frequency of occurrence of the isolates have Aspergillus>Penicillium while Microsporium, Fusarium and Acremonium are the same. The unique ability of these isolates to adapt to such conditions of petroleum hydrocarbon content in soil can be effectively used in bioremediation of oil impacted areas in the Niger Delta.Keywords: Mycoremediation, Hydrocarbon, Aspergillus, bioremediation, Impacted, Delta
Highlights
Mycoremediation is a form of bioremediation in which contaminated sites are converted into less contaminated ones by the use of fungal mycelium (Bennet et al, 2002)
Mycoremediation technique has been applied to oil spill contaminated and polluted soil, industrial chemicals, contaminated water and even farm waste (Bennet et al, 2002).The cleanup of a contaminated site requires a consortium of microorganisms; bacteria as well as fungi
Fungi mycelia penetrate oil, increasing surface area for biodegradation and bacteria attack (Chaillanet al., 2004).This presence study is aim at determining the physiochemical properties of soil sample and identifying the total heterotrophic and hydrocarbon utilizing fungi from Ejamah-Ebubu oil spill site located at Eleme Local Government Area in Rivers state Nigeria
Summary
About 0.1 ml of the 10-3 and 10-4 dilution of each sample was spread on the surface of Potato dextrose agar (PDA) into which 0.1 ml of lactic acid was added and incubated at 280C for 5 days. Total Hydrocarbon Utilizing Fungal Count: About 0.1 of 10-3 and 10-4 dilution were spread onto the surface of freshly prepared acidified mineral salt medium which contain in g/l Characterization, spore staining and identification of Hydrocarbon utilizing Fungi: The method of Bennet (2002) was adapted for characterization and identification This include macroscopic examination and microscopic examination. Preparation of Fungi Innoculum and Biodegradation of crude oil by fungi isolate: Fungi isolates were innoculated into 100 ml of Potato dextrose broth into which 0.1 ml of lactic acid is added. Procedure had been described in previous research work (Akomah and Abu, 2015)
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