Abstract

Pea protein dry-fractionated (PDF), pea protein isolated (PIs), soy protein isolated (SIs) and oat protein (OP) were combined in four mixes (PDF_OP, PIs_OP, PDF_PIs_OP, SIs_OP) and extruded to produce meat analogues. The ingredients strongly influenced the process conditions and the use of PDF required higher specific mechanical energy and screw speed to create fibrous texture compared to PIs and SIs. PDF can be conveniently used to produce meat analogues with a protein content of 55 g 100 g−1, which is exploitable in meat-alternatives formulation. PDF-based meat analogues showed lower hardness (13.55–18.33 N) than those produced from PIs and SIs (nearly 27 N), probably due to a more porous structure given by the natural presence of carbohydrates in the dry-fractionated ingredient. PDF_OP and PIs_PDF_OP showed a significantly lower water absorption capacity than PIs OP and SIs_OP, whereas pea-based extrudates showed high oil absorption capacity, which could be convenient to facilitate the inclusion of oil and fat in the final formulation. The sensory evaluation highlighted an intense odor and taste profile of PDF_OP, whereas the extrudates produced by protein isolates had more neutral sensory characteristics. Overall, the use of dry-fractionated protein supports the strategies to efficiently produce clean-labeled and sustainable plant-based meat analogues.

Highlights

  • The world population is expected to reach 9.7 billion by 2050 [1], causing an increase in protein demand

  • Process conditions were strongly dependent on the raw materials and the use of dry-fractionated protein required a more intense specific mechanical energy (SME) to create fibrous texture compared to protein isolates

  • Results proved that dry fractionated protein concentrates can be conveniently used for the production of meat analogues in spite of lower protein content than protein isolates

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Summary

Introduction

The world population is expected to reach 9.7 billion by 2050 [1], causing an increase in protein demand. Meat and dairy products are the primary protein source for the most of population of developed countries, with rising average consumption at a global level, but they require a considerable amount of resources such as arable lands, pastures, feed for livestock and water [2]. This leads to concerns regarding the affordability of feeding the world without damaging the environment [3]. Plant-based protein alternatives are already established and available on the market and are mainly derived from soy (e.g., tofu and tempeh) or wheat gluten (e.g., seitan).

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