Abstract
Background: Dooshivishari Agad (DA) is an herbal formulation that has been documented in Indian Traditional Medicine as a remedy for latent poisoning. Objective: this research was conducted to study Physicochemical parameter & Phytochemical analysis of DA by HPLC. Methods: It includes authentication of raw drugs and preparation of DA. Physicochemical parameter was tested by Loss on drying ,Acid insoluble Ash, Water soluble ash, Total ash value, Water & Alcohol soluble Extractive Value. HPLC was used for phytochemical screening. Observation: In analytical study obtained values are Loss on drying 2.54%,Water soluble Ash 1.84%, Acid insoluble Ash 0.5% , Total Ash value 5.20% Water Soluble Extractive Value 16.89%, Acid Soluble Extractive Value 23.94%, pH 5.6.Preliminary data of phytochemical screening revealed that it is rich in phenol compound and terpenoids Conclusion: This study provide the data for standardisation & phytochemical characterisation of DA for its pharmacological action.
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