Abstract
In this study, two eco-friendly methods for agar extraction, enzyme-assisted extraction and enzyme extraction, were developed to promote workers’ health and provide environmental protection. The physicochemical properties of enzyme-assisted extracted agar (EAA) and enzyme-extracted agar (EA) were evaluated and compared with those of the traditional alkali-extracted agar (AA). Compared with EAA and EA, AA showed most ideal performances in the aspect of gel strength (1521 ± 54.5 g/cm2), sulfate content (0.84% ± 0.15%), and 3,6-anhydro-l-galactose content (29.2% ± 0.3%) (p < 0.05). EAA showed similar properties to and lower sewage discharge than AA; thus, enzyme-assisted extraction could be a promising alternative to the traditional method. Enzyme extraction supports sustainable development because this process requires less energy and generates fewer wastes than alkaline processes while offering advanced and promising features, including high viscosity, sulfate content, water retention, and low melting temperature.
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