Physico-chemical and biological activity of Malaysian channa striatus water extract on human foetal lung cell (IMR-90)

Abstract

Event Abstract Back to Event Physico-chemical and biological activity of Malaysian channa striatus water extract on human foetal lung cell (IMR-90) Winnie N. Shy Yi1, Syazwan A. Abdul Mutalib1, Badrul H. Yahaya2 and Saiful Zubairi1* 1 Universiti Kebangsaan Malaysia , School of Chemical Sciences and Food Technology, Faculty of Science and Technology , Malaysia 2 Universiti Sains Malaysia, Regenerative Medicine Cluster, Advanced Medical & Dental Institute (AMDI), Malaysia Local fishes in Malaysia including Channa species, particularly Channa striatus (haruan) have been traditionally used for wound healing and reducing pain. For that reason, this study was carried out to determine the Channa species water extract physico-chemical and its bioactivity on human foetal lung cell (IMR-90). Channa striatus (haruan) was used in this study. The sample was trimmed carefully to obtain the maximum fish fillet and extracted by using normal soaking extraction method (fish weight-to-solvent ratio = 1:4) as to procure the haruan aqueous extract. The extract was characterized for its physico-chemical properties such as pH, moisture content, rheological properties (n = 3), amino acid composition and fatty acid determination (arachidonic acid) (n = 2). The effective concentration (EC50) of the extract was conducted via 2-dimensional (2-D) culturing system on a normal human fibroblast cell (IMR90). The essential fish’s essence physical properties are as followed: 1) pH - 6.34 ± 0.01, 2) 97.3 ± 0.15% of moisture content, 3) total dissolved solid - 2.7 ± 0.01 (%, w/w) and 3) viscosity of 1.50 ± 0.31 mPa.s. The extract contained essential amino acid, glycine and fatty acid (arachidonic acid), which could aid in the process of wound healing despite their low concentration. However, the presence of those compounds have shown insignificant growth as compared to the control (without treatment) (p>0.05). Moreover, the immobile and irresponsive IMR90 growth rate on different extract concentrations was consistent with the absence of mortality throughout the 3-day incubation period (p>0.05). For that reason, the extract EC50 values could not be determined conceivably due to the high fish weight-to-solvent ratio which eventually deliver extremely low concentration of bio-active compounds for the cell-induced proliferation. Figure 1 Figure 2 Figure 3 Acknowledgements The authors would like to thank the Ministry of Higher Education (MOE) and Ministry of Science, Technology and Innovation (MOSTI), Malaysia, for providing financial support to this project (FRGS/2/2013/TK04/UKM/03/1 and 06-01-02-SF1271). Keywords: Wound Healing, Aqueous extract, Physico-chemical properties, Channa striatus, 2-D cell culture Conference: 6th Malaysian Tissue Engineering and Regenerative Medicine Scientific Meeting (6th MTERMS) 2016 and 2nd Malaysian Stem Cell Meeting, Seberang Jaya, Penang, Malaysia, 17 Nov - 18 Nov, 2016. Presentation Type: Poster Topic: Natural Products in Regenerative Medicine Citation: Shy Yi WN, Abdul Mutalib SA, Yahaya BH and Zubairi S (2016). Physico-chemical and biological activity of Malaysian channa striatus water extract on human foetal lung cell (IMR-90). Front. Bioeng. Biotechnol. Conference Abstract: 6th Malaysian Tissue Engineering and Regenerative Medicine Scientific Meeting (6th MTERMS) 2016 and 2nd Malaysian Stem Cell Meeting. doi: 10.3389/conf.FBIOE.2016.02.00009 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. 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Received: 08 Dec 2016; Published Online: 19 Dec 2016. * Correspondence: Dr. Saiful Zubairi, Universiti Kebangsaan Malaysia, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Bangi, Selangor, 43600, Malaysia, saiful-z@ukm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Winnie N Shy Yi Syazwan A Abdul Mutalib Badrul H Yahaya Saiful Zubairi Google Winnie N Shy Yi Syazwan A Abdul Mutalib Badrul H Yahaya Saiful Zubairi Google Scholar Winnie N Shy Yi Syazwan A Abdul Mutalib Badrul H Yahaya Saiful Zubairi PubMed Winnie N Shy Yi Syazwan A Abdul Mutalib Badrul H Yahaya Saiful Zubairi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.