Abstract

trans-Resveratrol (RSV) is a natural phenolic molecule of the stilbene family known for its anti-oxidant properties in the field of nutraceuticals and cosmetics. Its production by grapevine cell suspensions is induced by the addition to the culture medium of elicitor compounds, methyl jasmonate (MeJA) and cyclodextrins (CDs). Physico-chemical studies were performed to understand the mechanism of action of CDs on this bioproduction of RSV. Inclusion complexes of RSV in CDs were first observed and then interactions with MeJA were identified using various analytical techniques such as UV and nuclear magnetic resonance (NMR) spectroscopies, mass spectrometry (MS) and isothermal titration calorimetry (ITC).

Highlights

  • Known to be involved in plant defense mechanisms, transresveratrol or 3,5-40 trihydroxy-trans-stilbene, RSV, (Fig. 1a) and its derivatives, so-called viniferins, have been shown to protect grapevine species or cultivars against fungi such as downy and powdery mildew.[1,2] its extension of the lifespan of different organisms has been described and RSV is renowned as the major agent responsible for the French Paradox, based on the notion that daily consumption of red wine is good for human health

  • RSV is in high demand, and one way to meet the demand is through extraction from plant natural sources as roots of Fallopia japonica[5] or grape canes.[6]

  • Study of CD/RSV interactions First, we studied the interactions between various CDs used to elicit grapevine cell cultures and RSV to compare our results with previous data in the literature

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Summary

Introduction

Known to be involved in plant defense mechanisms, transresveratrol or 3,5-40 trihydroxy-trans-stilbene, RSV, (Fig. 1a) and its derivatives, so-called viniferins, have been shown to protect grapevine species or cultivars against fungi such as downy and powdery mildew.[1,2] its extension of the lifespan of different organisms has been described and RSV is renowned as the major agent responsible for the French Paradox, based on the notion that daily consumption of red wine is good for human health. The other protons, H1, H2, H4, and H6, are directed towards the outside This cavity has supramolecular inclusion properties in aqueous solution.[13,14] Some chemical derivatives of CDs, such as DIMEB (2,6-di-Omethyl-b-CD) and RAMEB (randomly methylated-b-CD), are available and exhibit inclusion properties despite signi cant differences in hydrophobicity, solubility, and conformational exibility.[15]. The strategy consisted of rst considering both CD/RSV and CD/MeJA interactions independently and evaluating the case in which the three compounds could interact with each other To probe these interactions, physico-chemical studies were undertaken by UV, MS, NMR and ITC. CD/RSV interactions were investigated using phase solubility diagrams, which revealed that RAMEB and b-CD were the best to solubilize RSV in water This is in agreement with biological results and was con rmed by MS studies. In agreement with the physico-chemical studies it was demonstrated that RAMEB and b-CD protect RSV from degradation

Results and discussion
Materials and methods
Conclusions
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