Physico-chemical studies of elastic compliance and adsorption of DOPC vesicles and its mixture with charged lipids at fluid/solid interface

Abstract

Lipid bilayer mechanics is crucial to membrane dynamics and in design of liposomes for delivery applications. In this work, vesicles of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) (size from 50 nm to 1 μm) and its mixtures with anionic 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (DOPG) and cationic dimethyldioctadecylammonium bromide (DODAB), have been studied under shear stress at fluid/solid interface and their elastic compliance evaluated. Results show that the rate of spreading of the smaller vesicles (∼70 nm) is about 1.4 times slower than those of larger ones (∼1 μ) and that DOPC has the highest elastic compliance compared with DOPC + DOPG and DOPC + DODAB vesicles. A direct correlation between the elastic compliance and the size of the vesicles shows larger vesicles are more structurally labile during adsorption and subsequent adhesion to solid surfaces than the smaller ones. Specific role of bound water in DODAB is reflected in the lowest elastic compliance of DODAB compared to other lipids. Results show that during the process of adhesion at the fluid/air interface, the vesicles undergo contraction, thereby transmitting mechanical stresses to their microenvironment, which matches the SAXS electron density profiles that indicates larger vesicles have thicker bilayer membranes with larger volume of water compared to the smaller sized ones.