Physico-chemical, Spectrophotometric and cell-line Toxicity study of Isolated Biomaterial

Abstract

The aim of this research was to isolate and characterize the novel biopolymer from Cicer arietinum seeds. The biopolymer was isolated by simplified and economical process and analysed for different physico-chemical and spectral properties. The isolated biopolymer appears as free flowing powder. The isolation procedures were optimized by repeating it for six times and thus were found to be economical and reproducible in nature with the yield of 12±0.4%. The different functional groups like presence of hydroxyl, alkanes and alkenes, carboxylic acid which confirms its polymeric nature. The other groups like aromatic ring at, ketone were found to be present in the FTIR spectra, NMR and mass spectra. shows the mean % cell viability ranging from 101.62±0.928 to 81.9±6.265% with IC50 values (µg/ml) of >500. Thus the cell viability assay data demonstrate that there is no cell death observed in assay.The study finds that the isolated biopolymer from Cicer arietinum seeds contains unusual inherent stabilizer and cum retardant capabilities that can be exploited to develop a drug loaded delivery systems for long-term administration of drug candidate.