Abstract
The interfacial potential difference between the phases in Dextran 500—poly(ethylene glycol) 6000 and Ficoll 400—dextran 70 biphasic systems was measured as a function of the ionic composition of the systems. The results obtained are compared to those on the partitioning of dinitrophenyl amino acids and human red cells obtained previously. In many cases, there is no relation between the partitioning of charged solutes and particles and the potential difference measurable in the biphasic system. It is concluded that the distribution potential measurable in a given biphasic system as well as the ionic strength of the system generally cannot be used as a feature of the system generally important for the partitioning of solutes and particles in such systems. Two main features of the phases are assumed to be important for the partition process: (i) the hydrophobic properties of the phases and (ii) the hydration properties of the phases. An approach to estimate the latter is proposed.
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