Abstract
Although green macro-algae represent a renewable and highly abundant biomass, they remain poorly exploited in terms of carbohydrate polymers compared to red and brown ones and other lignocellulosic materials. In this study, cellulose from the green macro-algae Enteromorpha sp. was isolated, physico-chemically characterized and enzymatically functionalized. The cellulose content was about 21.4% (w/w). FTIR analyses indicated an absence of acetyl or uronic esters confirming the absence of hemicellulose contamination. The 36% crystallinity index of the extracted cellulose revealed a high amorphous character as determined by X-ray diffraction. The moisture adsorption isotherms and specific surface measurements were respectively 42.87g/100g and 8.34m2/g. The Enteromorpha sp. cellulose was first enzymatically saccharified by a commercial cellulase preparation from Aspergillus niger with a hydrolysis yield of 70.4%. Besides, it was successfully functionalized based on the transglycosylation mechanism of the same enzymatic preparation, to produce highly added-value biosurfactants (butyl-glucoside) with a concentration of 8mM.
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