Abstract
Rinderpest virus grown in Vero cells was purified by equilibrium sedimentation in potassium tartrate gradients. The characteristics of the virus and its RNA dependend on the passage history of the virus. Undiluted passage virus had a density ofc 1.21 g/ml in potassium tartrate and contained RNA sedimenting atc. 20S and 5S. Virus obtained by dilute passage had a density ofc. 1.24 g/ml and contained 50S in addition to 20S and 5S RNA. Both diluted passage and undiluted passage viruses contained polypeptides with molecular weights of 98, 79, 75, 66, 48, 43 and 37×103. Trace amounts of polypeptides with molecular weights of 29 and 20×103 were also detected. The molecular weights of the major polypeptides are similar to those of the polypeptides present in measles and canine distemper viruses.
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