Abstract

The reduction of speckle noise by physically changing the pupil of the imaging system, as first envisioned in optical holography, is experimentally applied to a digital holographic microscope (DHM). The imaging pupil of a DHM, operating in image plane telecentric-afocal architecture, is changed in a controlled way between successive recordings, allowing the shooting of multiple partially-decorrelated holograms. Averaging the numerically reconstructed holograms yields amplitude and/or phase images with reduced speckle noise. Experimental results of biological specimens and a phase-only resolution test show the feasibility to recover micron-sized features in images with reduced speckle noise.

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