Physical phase compensation in digital holographic microscopy by using of electrical tunable lens

Abstract

An electrical tunable lens is applied in digital holographic microscopy for physical spherical phase compensation. When different microscope objectives are applied to one digital holographic microscope, the physical spherical phase compensation needs different reference wavefronts. The focal length of the electric tunable lens can be adjusted by applying different voltages. We have measured the morphology changes of the tunable lens under different voltages. According to the measurement, the tunable lens has the capability to change wavefront via changing of the applied voltages. Thus we apply the tunable lens in the digital holographic microscope with multiple microscope objectives to fulfill the physical spherical phase compensation. The measurement results for the tunable lens together with the phase compensation results are presented.