Abstract
We have described various physical methods, which have been employed in the determination of three dimensional structures of biological molecules. The basic principles of methods currently in use, have been described. Principles of two major techniques, namely NMR and X-ray crystallography have been discussed in detail. Applications of such techniques, to study large biological molecules and intermolecular interactions between such molecules have been described. Molecular assemblies, containing more than one molecule and complex structures such as ribosomes have been discussed.
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