Abstract
Marker-rescue experiments with λ-P4 hybrid phages constructed in vitro have identified the locations of two P4 genes: the transactivation gene (δ) is in the left 10% of the genome, and the DNA replication gene (α) lies between 10 and 70% from the left end of the P4 genome. A λ-P4 hybrid phage which carries all of the essential genes of λ and the left 70% of the P4 genome can produce a P4-like phage which is indistinguishable in phenotype from the parental P4 vir1 phage. One of these P4-like phages has a P4-sized genome which is 70% homologous to P4 and produces λ-specific RNA. The existence of this phage and of two P4 deletion mutants suggests that the right 30% of the P4 genome contains no essential genes.
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