Abstract
Rifampicin has been shown to inhibit the maturation of poxviruses at a discrete step in envelope formation ( Moss et al, 1969; Pennington et al, 1970; Nagayama et al., 1970 ; Grimley et al., 1970 ). A rifampicin-resistant vaccinia virus mutant (Rif R) was selected for its ability to grow in the presence of 100 μg/ml of rifampicin. Utilizing intact DNA or endonuclease restricted cloned DNA subfragments derived from the Rif R mutant virus, the locus specifying rifampicin resistance was physically mapped by marker rescue analysis leftward of the unique XhoI site within the HindIII D fragment. DNA sequencing of a 445 by fragment encompassing this region revealed an AT to GC transition when compared with the equivalent wild-type DNA fragment. Analysis of the six potential open reading frames within the 445-bp fragment indicated only one available open reading frame. On this basis, the rifampicin-resistant vaccinia virus mutant was shown to have a codon transition from asparagine to aspartic acid.
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