Abstract
A cosmid contig was constructed from a YAC clone with a 220-kb insert that spans the centromeric side of the human MHC class II region, corresponding to the mousetcomplex. The gene order was identified to beHSET–HKE1.5–HKE2–HKE3–RING1–HKE6–HKE 4 (RING5).The genomic sequence of a 42,801-bp long region encoded by one cosmid clone in theRING1, HKE6,andHKE4subregions was determined by the shotgun method. The exon–intron organization of these three genes,RING1(Ring finger protein),HKE6(steroid dehydrogenase-like protein), andHKE4(transmembrane protein with histidine-rich charge clusters), was determined. The previously reportedRING2gene was revealed to be identical toHKE6.Transcripts fromHKE4were detected in the placenta, lung, kidney, and pancreas. Those ofHKE6were found in the liver and pancreas. The 25-kb region proximal to theRING1gene includes an extensive dense cluster ofAlurepeats (about 1.2Aluper kb), and no gene has been identified in this so far. The region is equivalent to part of the mousetcomplex and could be of relevance to human development.
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