Abstract
Rye (Secale cereale L.) 4R chromosome contains elite genes that are applicable for wheat (Triticum aestivum L.) cultivar improvement. PCR-based 4R-specific markers can benefit the detection of elite genes on 4R in wheat backgrounds. In this study, a new fluorescence in situ hybridization (FISH) map of the 4RKu chromosome of rye Kustro has been constructed. A set of 4RKu dissection lines was obtained and 301 new 4RKu-specific markers were developed using specific length amplified fragment sequencing (SLAF-seq) technology. These markers were combined with the 99 4RKu-specific markers previously developed, and were physically mapped to 4RKu chromosome using the new FISH map and the 4RKu dissection lines. A total of 338 of the 400 markers have been successfully mapped to six regions of 4RKu chromosome. Additionally, the powdery mildew resistance gene(s) on the 4RLKu arm was located to the segment between L.4 and L.8, the same region where 115 4RLKu-specific markers were mapped. The markers developed in this study can be used to identify a specific segment of 4R chromatin in wheat backgrounds, help construct a high-density physical map of 4R chromosome, and facilitate the utilization of elite genes on 4R chromosome in wheat breeding programs.
Highlights
Rye (Secale cereale L.) is an important gene source for wheat (Triticum aestivum L.) cultivar improvement
The irradiated MK seeds were used as recipients to cross with common wheat T. aestivum L
Oligo-pSc119.2-1 produced a strong signal at the interstitial site of 4RLKu, and Oligo-pSc200 and Oligo-pSc250 produced clear signals at the sub-telomeric region of 4RLKu (Figure 1)
Summary
Rye (Secale cereale L.) is an important gene source for wheat (Triticum aestivum L.) cultivar improvement. The short arm of rye chromosome 1R has been widely used to develop wheat cultivars through 1BL.1RS or 1AL.1RS translocation chromosomes (Berzonsky et al, 1991; Rabinovich, 1998; Kumar et al, 2003; Landjeva et al, 2006). Other rye chromosomes contain elite genes that can be used for wheat improvement. 4R chromosome contains disease- and insect-resistance genes. Lukaszewski et al (2001) reported that the 4RL arm of Secale montanum Guss carried a Russian wheat aphid (RWA) resistance gene. New powdery mildew resistance gene(s), which differ from previously reported rye genes, were located on 4R
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