Abstract
The relationship of the phage P1 chromosome to the chromosome in lysogenic bacteria has been investigated. 32P-labeled bacterial DNA isolated from logphase Escherichia coli K12 W3110, either lysogenic or non-lysogenic for phage P1kc, was sedimented through a sucrose gradient (5 to 20%) and fractions were removed for DNA-DNA hybridization on P1 DNA-agar. Approximately 2% of any fraction tested was specifically bound to P1 DNA. The binding specificity was demonstrated by using both W3110 and W3110 (P1) DNA, and by the interference exhibited by P1 DNA, and not W3110 DNA, for the binding of W3110 (P1) DNA to P1 DNA-agar. The finding that a constant percentage of the W3110 (P1) DNA, recovered from any part of the sucrose gradient binds specifically to P1 DNA is interpreted to mean that the P1 prophage DNA is an integrated part of the bacterial chromosome. The approximate 2% specific hybridization suggests that there is one P1 prophage associated with each chromosome in a lysogenic bacterium.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callSimilar Papers
More From: Journal of Molecular Biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
