Physical characterization and uptake of iron oxide nanoparticles of different prostate cancer cells

Abstract

Abstract Among nanoparticles, magnetic nanoparticles are the most appealing candidate for diagnosis and cancer therapy. The researchers are tempting to improve the particles properties, including the size, shape, coating, and magnetic behavior or heating characteristics. Core shell type of magnetic nanoparticle is an important property that modulates their internalization via normal and cancer cells. In this study, magnetite nanoparticles (MNP) covered by N-(2-aminoethyl)-3-aminopropyltriethoxysilane (aminosilane – APTES) were synthesized by co-precipitation of aqueous solution of ferric chloride and ferrous sulfate iron salts with ammonium hydroxide as a base and functionalized by APTES to increase the viability and affinity of the particles to the cancer cells. The structural and morphological properties of these particles were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transformed infrared spectroscopy (FTIR). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT assay) was carried out, to check the viability of the cells treatment with MNP and APTES-MNP. To study the cellular uptake in vitro, two prostate cell lines were investigated: PC3 as a cancerous cell line and BPH1 as a benign epithelial cell line (normal cells). Both cell lines were incubated for 24 h with different concentrations of MNP and APTES-MNP (100 and 500 μg/ml and one untreated sample as control). TEM and flow cytometry (FC) analyses were subsequently carried out to monitor the cellular uptake of MNP and APTES-MNP. FC data revealed an increase in cell granularity following the treatment with high concentration of the particles. Data showed that PC3 cancer cells take up more APTES-MNP with respect to control cells than BPH1 benign cells and in contrast BPH1 cell uptake MNP correlated to control cells more efficient than PC3 cells. The results from FC and TEM analyses demonstrate increasing of affinity of particles to cancer cell line (PC3). In this project we investigated the effect of surface functionalization of NP to affinity of the MNP and APTES-MNP on PC3 cells as a malignancy prostate cell and BPH1 benign cells as a normal cells. This approach may help to optimize the efficiency of hyperthermia for prostate cancer through internalization of particles to the cells or attaching to the membrane.