Abstract
The sensitivity of various genetic marker pairs to separation by shear indicates a non-permuted linear structure for the DNA molecule of SP82 phage which is co-linear with the known genetic map. When a solution of SP82 DNA is stirred the initial majority component, 63 s, is broken to 47 s, and at higher speeds to 33 s. Isolated 63 s, 47 s and 33 s molecules are capable of introducing single genetic markers into competent Bacillus subtilis. Comparison of the relative effectiveness of 47 s and 63 s molecules in introducing various linked marker pairs into the cell reveals that markers spanning one region of the genome are unlinked by the first shearing event. This region is close to the center of the genetic map. Examination of the relative activities of 33 s and 47 s molecules suggests two new regions of shear sensitivity. These regions occur on either side of the first shear site and are qualitatively in agreement with the positions expected for “quartering” shear events.
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