Abstract
The objective of this study was to evaluate the quality of water purification system and identify the bacteria this system, predict bacterial adherence according to the hydrophobicity of these microorganisms and of the polypropylene distribution loop for purified water. The assessment of drinking water that supplies the purification system allowed good-quality physical, chemical, and microbiological specifications. The physicochemical specifications of the distributed purified water were approved, but the heterotrophic bacteria count was higher than allowed (>;2 log CFU mL-1).The sanitation of the storage tank with chlorine decreased the number of bacteria adhered to the surface (4.34 cycles log). By sequencing of the 16SrDNA genes, six species of bacteria were identified. The contact angle was determined and polypropylene surface and all bacteria were considered to be hydrophilic, and adhesion was thermodynamically unfavorable. This case study showed the importance of monitoring the water quality in the purified water systems and the importance of sanitization with chemical agents. The count of heterotrophic bacteria on the polypropylene surface was consistent with the predicted thermodynamics results because the number of adhered cells reached approximate values of 5 log CFU cm-2.
Highlights
To become suitable for use in the laboratory or in industry pharmaceutical, water should be treated to eliminate its contaminants, creating purified water
The results show that during the 40 days of analysis, for 23 days, the number of bacteria was above the allowed amount (2 log CFU mL-1 or 100 CFU mL-1)
It was observed that the adhesion between the polypropylene and the isolated bacteria was thermodynamically unfavorable
Summary
To become suitable for use in the laboratory or in industry pharmaceutical, water should be treated to eliminate its contaminants, creating purified water. The entire process of production, storage, and distribution should be properly validated and monitored for significant physical-chemical and microbiological characteristics. Monitoring water purification systems is critical to obtain water with good microbiological and physicalchemical characteristics, and the assessment of water should meet the technical recommendations and the requirements of legislation. The quality of purified water is an important concern for use in analytical laboratories, As a fundamental guideline, potable water is the starting point for any process of purification (FDA, 1986). The physical-chemical quality is related to the fundamental assessment of the water for alkalinity, conductivity, residual free chlorine, hardness, ISL (Langelier Saturation Index), pH, and silica content. Equipment surfaces of the systems are susceptible to adhesion and biofilms can be established as a source of viable microorganisms in purified water (Penna, Martins, Mazzola, 2002). It was reported that purified water, even with total organic carbon
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