Physical and biological control of protistan community composition, distribution and abundance in the seasonal ice zone of the Southern Ocean between 30 and 80°E

Abstract

Abstract Protists are critical components of the Antarctic marine ecosystem as they comprise most of the living carbon and are the base of the Antarctic food web. They are also key determinants of vertical carbon flux and mediate draw-down of atmospheric CO 2 by the ocean. The community composition, abundance and distribution of marine protists (phytoplankton and protozoa) was studied during the Baseline Research on Oceanography, Krill and the Environment-West (BROKE-West) survey, in the seasonal ice zone during the 2005-2006 austral summer between 30°E and 80°E. Light and electron microscopy were used to determine the protistan composition and abundance in samples obtained at 30 sites from surface waters and at 26 sites from the depth of the maximum in situ chlorophyll fluorescence (Chl max). Cluster analysis was used to identify 5 groups of sample sites at the surface and 5 at the Chl max that were of similar protist composition and abundance. The physical characteristics, taxonomic composition, indicator taxa, and taxonomic diversity were determined for each group. In the southwest, a bloom of colonial Phaeocystis antarctica dominated the protistan community composition and biomass amongst the receding ice, but this was replaced by the flagellate life stage/s of this haptophyte in waters to the north. In the southeast, a diatom bloom had the highest diversity of protist taxa observed during the survey and centric diatoms dominated the biomass. Outside these blooms, grazing by krill probably reduced the composition and abundance of large diatoms and autotrophic dinoflagellates in coastal to mid-inshore waters. Only in offshore waters did large diatoms and dinoflagellates increase in abundance and diversity, despite low concentrations of iron and silicate at many of these sites. This increase was probably due to reduced top-down control by krill and other large zooplankton. Large diatoms dominated in offshore waters, despite other coincident studies showing that the trophic structure and function of the microbial community was frequently typical of nanoflagellate-dominated systems in high-nutrient low-chlorophyll waters. Nanoflagellate abundances were low during the survey and were either poorly resolved by our study or limited by microheterotrophic grazing. We propose that protistan abundance and composition in the sea-ice zone of the Indian Sector were determined by synoptic-scale oceanographic features, meso-scale changes caused by sea-ice retreat and meso- to nano- scale interactions between grazers and the composition and abundance of their protistan prey.